Molecular Biology A
Code
10654
Academic unit
Faculdade de Ciências e Tecnologia
Department
Departamento de Ciências da Vida
Credits
6.0
Teacher in charge
Ana Madalena Peres de Drumond Ludovice Mendes Gomes, Rosario Mato Labajos
Weekly hours
3
Total hours
48
Teaching language
Português
Objectives
The general objective of the C.U. is to provide a comprehensive overview of the key concepts in molecular biology and concepts and uses of methods and techniques in molecular biology.
Prerequisites
To students of the course in Cell and Molecular Biology. Although not required, completion of previous C.U. of this programme, such as ‘Biochemistry’, ‘Laboratory Techniques I’ and ‘Cellular Biology’ is likely to be exceptionally helpful.
Theoretical/practical lectures attendance is mandatory to obtain frequency.
Subject matter
Topics to be covered in theoretical lectures include nucleic acids structure and function- DNA replication, gene and genome structure, transcription, translation, the genetic code, mutation and DNA repair, and regulation of gene expression, with particular focus in prokaryotes (lac and trp operons of E.coli). Gene regulation of lambda phage.
Theoretical- practical sessions focus the basic techniques for nucleic acids isolation, purification and analysis. The fundamental concepts and application of each technique are described.
Bibliography
Benjamin Lewin (2007). Genes IX. Jones and Bartlet Publishers.
http://biology.jbpub.com/book/genes/
Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter (2008. Molecular Biology of the Cell. Garland Science
http://www.scibooksdirect.com/covers/Misc_docs_1161312.pdf
Harvey Lodish; Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott (2012). Molecular Cell Biology. W.H. Freeman
http://whfreeman.com/Catalog/product/molecularcellbiology-seventhedition-lodish
Sambrook J., and D. W. Russell. (2001) Molecular Cloning - A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press. New YorK
Teaching method
Theoretical classes and theoretical-practical lectures where the fundamentals of molecular biology methodologies and techniques are introduced.
Evaluation method
The theoretical and practical-theoretical contents are evaluated by two written tests (70% of final grade) performed individually and 4 quizzes (20% of final grade) performed by groups of students independently and online (moodle) and oral presentation (10% of final grade)