Molecular Diagnostics
Code
10915
Academic unit
Faculdade de Ciências e Tecnologia
Department
Departamento de Ciências da Vida
Credits
3.0
Teacher in charge
José Paulo Nunes de Sousa Sampaio, Maria Alexandra Núncio de Carvalho Ramos Fernandes
Weekly hours
4
Total hours
35
Teaching language
Português
Objectives
The objective of this course relates to the knowledge of the technologies applied to genetic diagnosis either in area of molecular biology or modern cytogenetics. It is intended that all students understand the processing of a biological sample from the entrance into a laboratory, their analysis and results interpretation. New technologies will also be addressed in the area of nanobiotechnology for detection of nucleic acids.
Prerequisites
Labs mandatory, and written reports. Participation in problem solving sessions.
Theme based seminars.
Subject matter
Theoretical
Molecular Diagnostics
1.Genetic Test
Prenatal Diagnosis
Molecular Cytogenetics
Biological samples: harvesting and processing.
Molecular Biology Laboratory
1.Molecular Biology Techniques in Diagnostics
2.Strategies in Genetic Diagnostics
3.New Technologies for Genetic Diagnosis
4.Aplications
Lab
Fusion transcripts detection in CML
Bibliography
Molecular Diagnostics: Fundamentals, Methods and Clinical Applications
Lela Buckingham, Maribeth L. Flaws
F.A Davis Company, 2011
Molecular Diagnostics: Techniques and Applications for the Clinical Laboratory
Wayne W. Grody, Robert M. Nakamura, Frederick L. Kiechle, Charles Strom
Academic Press, 2010
Molecular Diagnostics: For the Clinical Laboratorial
William B. Coleman, Gregory J. Tsongalis
Humana Press, 2006
Fundamentals of Molecular Diagnostics,
David E. Bruns MD, Edward R. Ashwood MD, Carl A. Burtis PhD
Diagnostic Techniques in Genetics
Jean-Louis Serre
John Wiley and Sons, Ltd, 2006
Teaching method
Students will have at their disposal powerpoint presentations of the classes (different topics of discussion as well as scientific paper important to their formation).
Laboratorial classes: Protocols for early assessment of the work to be performed
Evaluation method
Theoretical Exam - 60% of the final grade (> 9.5 valores)
Laboratorial classes report - 15%
Seminars - 25%
Each component should have a grade above 9,5 to be approved.
Laboratorial classes are mandatory
After approval to the UC - exam to 100% of the final grade