Separation Processes for Biological Products
Code
10685
Academic unit
Faculdade de Ciências e Tecnologia
Department
Departamento de Química
Credits
6.0
Teacher in charge
Ana Cecília Afonso Roque, Isabel Maria Rola Coelhoso
Weekly hours
5
Total hours
81
Teaching language
Português
Objectives
In this course it is expected to give information on the separation techniques used in biotechnology, to provide students with sufficient information to formulate isolation schemes for a given biomolecule, and to design equipment for each unit operation. The study of alternative and innovative purification methods will also be stimulated through seminar presentation by the students. It is also envisaged to give students an overview of the exigencies in the handling, purification and validation of processes involving biological products, as the operating conditions differ markedly from chemical engineering.
Subject matter
- Introduction to Bioseparation Processes – Bioprocesses Classification; properties, Classification and Applications of Biological products; Production-Cost relation; RIPP Schemes and typical unit operations; Examples of industrial biochemical processes.
- Solid-Liquid Separations – Operations to remove solids: (i) Filtration at constant flow and pressure. Filter design. (ii) Sedimentation. Sedimentation velocity; Stokes´ Law; Design of sedimentation equipment. (iii) Centrifugation. Applied Stokes law, Equivalent area of sedimentation; types of centrifuges; Equations for centrifuge design.
- Cell disruption- Structure and composition of cell membranes and walls; Chemical and mechanical methods for cell disruption.
- Chromatography
- Adsorption - Adsorbents; Adsorption equilibrium; Solute movement theory; Local equilibrium theory; Packed bed adsorption; Mass transfer zone approach; Linear dispersion and staged models; Van Deemter equation; . Examples of industrial processes.
- Membrane Separations - Basic concepts; Membrane preparation and characterization; Modules; Transport in porous membranes: concentration polarization; Transport in nonporous membranes: solution-diffusion model; Membrane separation processes: pressure and concentration driven; Modules/configurations and applications.
- Integrated processes - Selection and sequencing of separations; Overview of separation methods; Energy, heuristics and environmental criteria; Analysis of specific bioprocesses (e.g., citric acid production; human insulin production).
- Validation of bioprocesses; Good Manufacturing Practices and validation of Biopharmaceutical Downstream Processing
Bibliography
“Bioseparations: Downstream Processing for Biotechnology”, Paul A. Betler, A.L. Cussler, Wei-Shou Hu, John Wiley & Sons (1988) USA
“Handbook of Downstream Processing”, Ed. Elliott Goldberg, Blackie Academic & Professional (1997) UK
“Bioseparations Science and Engineering”, Roger Harrison, Paul Todd, Scott Rudge, Demetri Petrides, Oxford University Press (2003) UK
“Bioprocess Engineering Principles”, Pauline Doran, Academic Press (2003), UK
"Industrial Bioseparations: Principles and Practice"; Daniel Forcitini, Wiley-Blackwell (2007)
Teaching method
The teaching unit on Separation Processes for Biological Products comprises of lectures and practical classes. In the practical classes, it is envisaged to solve data-handling problems related with the theoretical concepts introduced in the lectures, as well as to present and discuss scientific papers on the subject. Laboratorial classes are also programmed. The students will prepare seminars related with the topics of the course, giving emphasis to new techniques.