Analytical Bioorganic
Code
11265
Academic unit
Faculdade de Ciências e Tecnologia
Department
Departamento de Química
Credits
6.0
Teacher in charge
Elvira Maria Sardão Monteiro Gaspar
Weekly hours
3
Total hours
57
Teaching language
Português
Objectives
The main objectives are: 1) learning the application of different chromatographic techniques; 2) learning the sample preparation techniques; 3) understanding the quantitative analytical chemistry.
Prerequisites
Basic knowledge in Chemistryand Organic Chemistry.
Basic knowledge of statistics.
Subject matter
Instrumentation: HPLC, GC and GC-MS - basic components and how to use.
Sample preparation: SPE, SPME, liquid extraction, lyophilization, dryness and derivatization.
Analytical method validation.
Bibliography
Organic Laboratory Techniques, Ralph J. Fessenden, Joan S. Fessenden and Patty Feist, Brooks/Cole (Ed.), 2001.
Principles of Analytical Chemistry – A Textbook, M. Valcárcel, Springer-Verlag (Ed.), 2000.
Handbook of derivatives for chromatography; Karl Blau and Graham S. King (Ed.), Heyden and Son Ltd, London, 1978.
Handbook of Analytical Derivatization Reactions; Daniel R. Knapp, John Wiley & Sons Inc., New York, 1979.
Statistics for Analytical Chemistry; J.C. Miller and J.N. Miller, Ellis Horwood Limited, Chichester, England, 1988.
Teaching method
Theoretical subjects are presented and explained to students in lectures in computerized room so learning to be more interactive - view the contents of other prestigious institutions (1.5 h / week).
Each student has 5 practical (2h)classes, working in group: 1 GC work involving gas chromatography, liquid chromatography HPLC and more 3 works involving sample preparation: derivatization and analysis by GC-(FID/MS) and solid phase extraction (SPE) and analysis by HPLC-DAD and still solid phase microextraction and analysis-by SPME-GC (FID / MS).
The final evaluation of this course: 40% of theoretical (2 tests) and 60% practical component which includes oral presentation (PPT) of practical work and critical analysis of a scientific paper.
Evaluation method
2 written tests and a presentation on the experimental part of the course, along with the ongoing evaluation of experimental component .
In case of a negative evaluation will or lack thereof, will enter the theoretical evaluation, classification examination (written).
60% of theoretical evaluation (30% each test) and 40% on the practical component.
The assessment shall in each component, an integer - scale 0-20.
None of the components may have negative evaluation, ie, lower than 10 points.
The frequency discipline is mandatory. Derives 70% attendance in lectures and attendance at all practical classe