Bioanalytical Procedures
Code
11176
Academic unit
Faculdade de Ciências e Tecnologia
Department
Departamento de Química
Credits
6.0
Teacher in charge
Ana Maria de Jesus Bispo Varela Coelho, Marco Diogo Richter Gomes da Silva
Weekly hours
4
Teaching language
Português
Objectives
- To acquire knowledge and skills in Analytical Biochemistry and related procedures
- To know the bioanalytical methods (spectrophotometry, mass spectrometry, chromatography, electrophoresis, enzymatic and immunologic methods) used to quantify and/or characterize drugs and compounds in biological matrices.
- To be able to implement and develop bioanalytical methods.
- To know the validation parameters, perform validation plans and conclude on the validity of bioanalytical methods.
- To be familiar with the basic aspects of regulation, quality control and quality systems.
- To be able to search and critically interpret the literature in this field.
Subject matter
- Introduction to Analytical Biochemistry.
- Bioanalytical methods relevant for the quantification and/or characterization of drugs, metabolites and other compounds in biological matrices.
- Strategies for the development and implementation of bioanalytical methods.
- Validation of bioanalytical methods.
- Introduction to regulation, quality control and quality systems in the Clinical Biochemistry Laboratory
Bibliography
- F Settle. Handbook of Instrumental Techniques for Analytical Chemistry. Prentice Hall PTR, 1997.
- MM Cox, GN Phillips, Jr. Handbook of proteins – Structure, function and methods. Volume 2. Wiley, 2007.
- D Sheehan. Physical Biochemistry: Principles and Applications, 2nd Edition, Wiley, 2009
- F Garofol. Bioanalytical Method Validation, in Analytical Method Validation and Instrument Performance Verification (eds C. C. Chan, H. Lam, Y. C. Lee and X.-M. Zhang), John Wiley & Sons, Inc., Hoboken, NJ, USA. 2004