Analytical Bioorganic
Code
11265
Academic unit
Faculdade de Ciências e Tecnologia
Department
Departamento de Química
Credits
6.0
Teacher in charge
Marco Diogo Richter Gomes da Silva
Weekly hours
3
Total hours
53
Teaching language
Português
Objectives
The main objectives are: 1) learning the application of different chromatographic techniques; 2) learning the sample preparation techniques; 3) understanding the qualitative and quantitative analytical chemistry.
Prerequisites
Basic knowledge in Chemistryand Organic Chemistry.
Basic knowledge of statistics.
Subject matter
Instrumentation: HPLC, GC, GC/MS and 2D-GC - basic components and how to use.
Sample preparation: SPE, SPME, liquid extraction, lyophilization, dryness and derivatization.
Analytical method validation.
Bibliography
Organic Laboratory Techniques, Ralph J. Fessenden, Joan S. Fessenden and Patty Feist, Brooks/Cole (Ed.), 2001.
Principles of Analytical Chemistry – A Textbook, M. Valcárcel, Springer-Verlag (Ed.), 2000.
Handbook of derivatives for chromatography; Karl Blau and Graham S. King (Ed.), Heyden and Son Ltd, London, 1978.
Handbook of Analytical Derivatization Reactions; Daniel R. Knapp, John Wiley & Sons Inc., New York, 1979.
Statistics for Analytical Chemistry; J.C. Miller and J.N. Miller, Ellis Horwood Limited, Chichester, England, 1988.
Teaching method
Theoretical subjects are presented and explained to students in lectures (1h / week).
Practical lectures, 2.5h/week in a working group.
It will be involved: GC, HPLC (LC-UV, LC-FLD, LC-UV-Vis), sample preparation methods (including classic methods, SPE and SPME). Introduction to multidimensional methods (comprehensive GCxGC and heart-cut MD-GC)
Evaluation method
1 theoretical test(individualy): 20%
1 Oral presentation (group): 40%
1 Report in article format (group): 40%
In case of a negative weighted average final evaluation (less then 9.5 in 20) the final mark will be obtained through exam where the obtained mark will be considered as a whole (100%).
The frequency discipline is mandatory. Derives 70% attendance in lectures and attendance at all practical classe