Bacteriologia Médica
Code
21209
Academic unit
NOVA Medical School | Faculdade de Ciências Médicas
Credits
7
Teacher in charge
Prof. Doutor Miguel Viveiros Bettencourt
Teaching language
Portuguese
Objectives
To acquire knowledge, skills, abilities and methods of analysis applied to research and laboratory diagnosis in Medical Bacteriology.
At the end of this course, master students will be able to:
- process and transmit knowledge on the pathophysiology and diagnosis of infections caused by bacteria;
- master the fundamentals and principles of medical bacteriology and their applications in diagnosis and applied research;
- analyze and critically evaluate experimental protocols for the application of laboratory diagnostic methods in medical bacteriology;
- define scientific concepts from diverse sources of valid information;
- plan and conduct independent research, and laboratory protocols, respecting the requirements imposed by methodological own quality standards of the scientific method and laboratory diagnosis.
Prerequisites
n/a
Subject matter
- Clinical aspects, epidemiological and therapeutic bacterial infections.
- Characterization of the bacterial biological agent(s) (genomic characteristics, structural or physiological).
- Description of methods used to perform the bacteriological laboratory diagnosis (isolation, identification and antibiotic susceptibility).
- Complementary techniques that allow collecting data from taxonomic, systematic, epidemiological and possible biotechnological and environmental (bioinformatics, molecular taxonomy, typing of bacteria, fermenting technology, bioremediation) oriented applications.
Theoretical and practical lectures/classes: Processing of samples for bacteria isolation and culture techniques. Identification methods. Antibiotic susceptibility tests for bacteria.
Bibliography
1. Canas Ferreira, W.F., Sousa J.C.F. & Lima N. (Eds.) (2010). Microbiologia. Edições Lidel, Lisboa. ISBN 9789727575152.
2. Barroso H, MeliçoSilvestre A, Taveira N (Eds) (2014). Microbiologia Médica Volumes I e II, Edições Lidel, Lisboa. ISBN: 9789727575763.
3. Murray, P.R., Rosenthal, K.S., Pfaller M.A. (2013). Medical Microbiology. 7th Edition. ElsevierMosbySaunders, St. Louis, EUA. ISBN: 9780323086929.
4. Tille, P. Ed. (2014). Bailey & Scott's Diagnostic Microbiology. 13th Edition. ElsevierMosbySaunders,
St. Louis, EUA. ISBN: 9780323083300.
5. Murray P.R., Baron, E.J., Jorgensen J. H., Landry M. L., and. Pfaller M. A. (Editors) (2007). Manual of Clinical Microbiology. 9th Edition. American Society for Microbiology (ASM) Press, Washington, EUA. ISBN13: 9781555813710.
Teaching method
Lectures each corresponding to a bacteriology thematic and specific competence described in the program will be presented with a context-review of the state of art and knowledge. Formal expository lectures, based on the transmission of knowledge to the master student, adapted to the learning objectives. The master student should receive all the necessary information in order to accomplish the objectives and receive theoretical skills suited for the achievement of the objectives of the practical component the solving of the case-studies.
Theoretical and practical lessons Students will apply the knowledge gained in the theoretical lectures on practical case study exercises in order to stimulate deductive capabilities, critical analysis, and appropriate choice of analytical methodologies and summarize results to solve the case. It is intended the active participation of students by demonstrating real examples of problems and technical/scientific questions that the master student will have to face to solve the case-study.
Laboratory practice sessions, protocols and tutorials will be given in advance to manipulate materials, equipment and reagents, and to learn the basics of some techniques as well as the basics on laboratory safety and bioinformatics analysis. The teaching materials will be accompanied with biological material of our routine laboratory (biological samples, isolates, bacteria, nucleic acids, etc.) that the student will have to process for bacteriological analysis. The research and critical discussion of supporting scientific papers will be privileged to help finding the working hypothesis beneath the solution of the case-study, forcing the systematization of the analytic methods to use in order to understand, for each technique, its context and analytic objective in the context of a working hypothesis, to research their own independent conclusions based on their results obtained. Interactive and demonstrative methodologies will be also used, promoting self-discovery of the technical solutions for the case-study if placed and applied to the laboratory routine in bacteriology, involving the students in the process of teaching and learning, qualitative and quantitative analysis of science articles to ensure the achievement of the learning objectives.
Evaluation method
1) T: Theoretical lectures, based slide show, accompanied by indication of complementary bibliography (35.5 contact hours).
2) TP: Theoretical-practical lectures with introduction of case-studies for the application of methodologies discussed the cases of diagnosis / monitoring / characterization of infections the bacteria and characterization of its resistance profile to drugs (2 contact hours).
3) PL: Practical laboratory classes where processing of samples for bacterial isolation and culture techniques, classical identification methods and molecular biology methods as well as the execution of susceptibility tests will be carried out. (10.5 contact hours). Practical protocols adapted to the solving of the case-studies presented in the theoretical-practical lectures.
4) OT: Tutorial guidance for students, practical training and supervision.
5) Final assessment: theoretical exam of 50 multiple choice questions (5 options, only one correct) for 120
minutes.