Bacterial Molecular Genetics: New Concepts and Applications of the RNA Biology
Code
21216
Academic unit
NOVA Medical School | Faculdade de Ciências Médicas
Department
Genetics
Credits
6
Teacher in charge
Doutora Cecília Maria Pais de Faria de Andrade Arraiano
Teaching language
Portuguese
Objectives
- The course aims to achieve teaching basic and modern concepts of genetic engineering, RNA methodologies, and functional genomics;
- The course aims to provide the necessary tools for the students to be able to solve problems and to perform practical experiments in the laboratory.
Prerequisites
n/a
Subject matter
Part A: Basic Principles and applications of bacterial molecular genetics
#1 - Genomes and nucleic acids;
#2 Gene Expression;
#3 Molecular Cloning;
#4 Analysis of the cloned genes and its products;
#5 - Molecular Biotecnology in microbial systems;
Part B: New concepts and applications of RNA Biology
#6 The RNA World;
#7 Methods to study RNA metabolism;
#8 RNA synthesis vs. degradation;
#9 Processing/Degradation of the RNA as a mechanism of pós-transcriptional control of gene expression;
#10 Good things come in small packages the power of small RNAs;
#11 Fromomics to Synthetic BIology and Systems Biology;
#12 - Impacts of recent discoveries in RNA technology in the world.
Bibliography
The publications mentioned below plus specific articles on the topics lectured.
Current Opinion in Microbiology 2014Cell Regulation Section. Editors: Cecilia M. Arraiano and Gregory Cook.
O Mundo do RNA, Novos Desafios e Perspectivas Futuras. (Publicado em Janeiro de 2007) Coordinação: CM. ARRAIANO e AM Fialho, Editora Lidel.
Teaching method
Since this unit is aimed at understanding the general principles of bacterial genetics ad Posttranscriptional control of gene expression and RNA biology, the theoretical lectures and practical classes given in this unit are suitable for achieving the objectives.
Evaluation method
The evaluation will include an exam with questions referring to the theoretical and practical parts. The exam is composed of 40 questions (multiple choice) and will last for 2 hours. The students will give a seminar and write a report of the practical classes.
The final grade will correspond to:
10% - Evaluation based on the participation in the classes; 40% - report of the practical classes and oral presentation; 50% -written final exam.
The curricular unit and the Professors will also be evaluated by the students.