Human cytomegalovirus infections
Code
21227
Academic unit
NOVA Medical School | Faculdade de Ciências Médicas
Department
Medical Virology
Credits
6
Teacher in charge
Prof. Doutor Paulo Jorge Pereira Cruz Paixão
Teaching language
Portuguese
Objectives
- To understand the epidemiology, main clinical aspects , diagnosis and treatment of CMV infections;
- To know how to perform the main steps of the diagnosis of these infections.
Prerequisites
n/a
Subject matter
- Introduction to the diagnosis of CMV infections. Application of different techniques depending on the host and the clinical context (immunocompetent, immunocompromised, pregnant, newborn);
- Real time PCR (general concepts and specific application for CMV);
- Discussion of clinical and laboratory cases;
Culture: Observation of the characteristic cytopathogenic effects; Preparation of "shell vial inoculation, disclosure and notice thereof after incubation;
Antigenemia: separation and preparation of the slides; coloration and observation;
- PCR (techniquel "in house"): extraction, amplification and detection;
- Real time PCR: extraction, amplification, detection.
Bibliography
- Paulo Paixão, Sofia Almeida, Paula A. Videira, Dário Ligeiro, Teresa Marques. Screening of congenital cytomegalovirus infection by a real time PCR in urine pools. European Journal of Pediatrics, 2012; 171 (1): 125129.
- Kotton CN, Kumar D, Caliendo AM, Asberg A, Chou S, DanzigerIsakov L, Humar A. Updated international consensus guidelines on the management of cytomegalovirus in solidorgan transplantation. Transplantation. 2013;96(4):33360.
- Revello MG, Gerna G. Pathogenesis and prenatal diagnosis of human cytomegalovirus infection. J Clin Virol. 2004;29(2):7183.
Teaching method
The lectures are taught by teachers of curricular unit, depending on the respective specializations. The form of lecture, in which they will address issues considered essential by providing data to guide students to an individual study.
Laboratory practical classes will enable all students to practice the different methodologies used in the diagnosis.
Evaluation method
- Written examination finally multiple choice test of 20 questions (duration 30 minutes) - 50%;
- Oral presentation and discussion of topics by groups of two students. Themes are distributed in 1st class and the presentation of each theme has a duration of 20 minutes - 50%.