Methods for Characterization of Biomolecules
Code
11177
Academic unit
Faculdade de Ciências e Tecnologia
Department
Departamento de Química
Credits
6.0
Teacher in charge
Miguel Nuno Sepúlveda de Gouveia Teixeira, Pedro Manuel Henriques Marques Matias
Weekly hours
4
Total hours
57
Teaching language
Português
Subject matter
I-Biomolecules- Proteins; Carbohydrates, Lipids and Membranes; Nucleic Acids; Molecular Complexes and interaction of biomolecules.
II- Methodologies
1.Spectroscopic Techniques
a) UV-Visible spectroscopy;
b) Fluorescence spectroscopy;
c) Vibrational spectroscopies: Infra-red and Fourier Transform IR. Raman and Resonance Raman;
d) Electron Paramagnetic Resonance spectroscopy.
e) Nuclear Magnetic Resonance spectroscopy;
2. Mass spectrometry
3. Determination of 3D structures of biological macromolecules:
a) Structural determination by X-ray Crystallography (Crystallization; X-ray diffraction; calculation of electronic density maps). Structural information by SAXS.
b) Structural determination by Electronic Microscopy (EM).
Bibliography
Biophysical Chemistry: Part I,II e III , Charles R. Cantor and Paul R. Schimmel, W. H. Freeman
Principles of fluorescence spectroscopy, Joseph R. Lakowicz, Kluwer Academic : Plenum Publishers, 1999.
Biomolecular EPR spectroscopy, Wilfred R. Hagen. - Boca Raton : CRC Press, 2009
Electron paramagnetic resonance : elementary theory and practical applications , John A. Weil, James R. Bolton, John E. Wertz, John Wiley & Sons, Inc., 1993
Practical approaches to biological inorganic chemistry, ed. Robert R. Crichton, Ricardo O. Louro, Elsevier, 2013