Legionella Infections
Code
21226
Academic unit
NOVA Medical School | Faculdade de Ciências Médicas
Department
Medical Bacteriology
Credits
6
Teacher in charge
Prof. Doutor Paulo Jorge Pereira Cruz Paixão
Teaching language
Portuguese
Objectives
Understand the epidemiology, main clinical aspects, diagnosis and treatment of infection by Legionella Understand the importance of maintenance of equipment associated with the production of aerosols anddisease prevention measures.
Prerequisites
n/a
Subject matter
General characteristics and epidemiology of Legionnaires disease : clinical and therapeutic . Importance of early diagnosis.
Prevention and surveillance the mportance of maintenance of equipment associated with the production of Nosocomial Infection aerosols: definitions, prevention and surveillance measures.
Laboratory diagnosis: Culture of biological samples in BCYE a and GVPC. Search antigen in respiratory specimens by direct immunofluorescence. Antibodies in sera by indirect immunofluorescence. L.pneumophila sg1 antigen in urine, for immunochromatography. Analysis and interpretation of results Typing by molecular biology.
Cell Culture.
Study of gene expression.
Molecular biology in clinical diagnosis.
Bibliography
- Chasqueira MJ, Rodrigues L, Nascimento M, Ramos M, Marques T. Genetic diversity and evolutionary relationships among Legionella pneumophila clinical isolates, Portugal, 1987 to 2012. Euro Surveill. 2014;19(46).
- Shivaji T, Sousa Pinto C, SanBento A, Oliveira Serra LA, Valente J, Machado J, Marques T, Carvalho L, Nogueira PJ, Nunes B, Vasconcelos P. A large community outbreak of Legionnaires disease in Vila Franca de Xira, Portugal, October to November 2014. Euro Surveill. 2014;19(50):20991.
- Hilbi H, Jarraud S, Hartland E, Buchrieser C. Update on Legionnaires' disease: pathogenesis, epidemiology, detection and control. Mol Microbial. 2010;76(1):111.
Teaching method
The objectives of understanding the epidemiology, clinical and therapeutic aspects of Legionella infection, as well as the importance of maintenance of equipment associated with the production of aerosols and prevention of the disease will be taught during the lectures.
Diagnosis and typing require contact with the different laboratory methodologies, which will be guaranteed by practical classes in the laboratory.
Evaluation method
The lectures are taught by teachers of curricular unit, depending on the respective specializations. The form of lecture, in which they will address issues considered essential by providing data to guide students to an individual study.
Laboratory practical classes will enable all students to practice the different methodologies used in the diagnosis and typing.
Student assessment Written examination finally multiple choice test of 20 questions (duration 30 minutes) 30%.
Oral presentation and discussion of topics by groups of two students. Themes are distributed in 1st class and the presentation of each theme has duration of 20 minutes 70%.