Bacterial Molecular Genetics: New Concepts and Applications of the RNA Biology
Code
21216
Academic unit
NOVA Medical School | Faculdade de Ciências Médicas
Department
Genetics
Credits
6
Teacher in charge
Doutora Cecília Maria Pais de Faria de Andrade Arraiano
Teaching language
Portuguese
Objectives
Learn basic principles and applications of microbial molecular genetics.
To know concepts of the molecular biotecnology in microbial systems.
Understand the mechanisms involved in the regulation of the RNA levels in the cell.
Understand the synergies between structure and function of ribonucleases.
Understand the control mediated by regulatory RNAs ( noncoding small RNAs, CRISPR/Cas9 sistems, ribozymes, riboswitches, etc..).
To know several technologies based on Next Generation Sequencing (RNAseq, CLIPseq, ribosome profiling etc..).
To be familiar with the concepts and the impact of recent discoveries based in RNA technologies.
Prerequisites
n/a
Subject matter
Part A: Basic princicples and applications of bacterial molecular genetics.
1 Genomes and nucleic acids
2 Gene expression
3 Molecular Cloning
4 Analisis of the cloned genes and their products.
5 Molecular biotechnology in microbial systems.
Parte B: Novos conceitos e aplicações da biologia do RNA
6 The RNA world.
7 Methods to study RNA metabolism.
8 RNA síntesis vs. degradation.
9 Processing/Degradation of RNA as a mechanism for posttranscriptional control of gene expression.
10 Good things come in small packages the power of small RNAs
11 From omics to Systems Biology and Synthetic Biology.
12 Impacts of recent discoveries based on RNA technologies.
Bibliography
The publications mentioned below plus specific articles on the topics lectured.
Current Opinion in Microbiology 2014Cell Regulation Section. Editors: Cecilia M. Arraiano and Gregory Cook.
O Mundo do RNA, Novos Desafios e Perspectivas Futuras. (Publicado em Janeiro de 2007) Coordinação: CM. ARRAIANO e AM Fialho, Editora Lidel.
Teaching method
Since this unit is aimed at understanding the general principles of bacterial genetics ad Posttranscriptional control of gene expression and RNA biology, the theoretical lectures and practical classes given in this unit are suitable for achieving the objectives.
Evaluation method
The theoretical lectures are taught by teachers of curricular unit, depending on the respective specializations. In the lectures the teachers will address issues considered essential by providing data to guide students to an individual study.
In the practical classes the students will execute methods which were described in the lectures. The students will have the opportunity to give a seminar in proposed topics; this will revise and enlarge the program of the lectures.
Evaluation of the students - 10% - Continuous evaluation based in the participation of the students in the classes., 40% - report of the practical classes and evaluation of the oral seminar, 50% - final written exam (the exam will have multiple choice questions).
The curricular unit and the lecturers will also be evaluated by the students.