General Biochemistry B
Code
10419
Academic unit
Faculdade de Ciências e Tecnologia
Department
Departamento de Química
Credits
6.0
Teacher in charge
Mário Emanuel Campos de Sousa Diniz
Weekly hours
4
Total hours
57
Teaching language
Português
Objectives
Aims:
After the course it is expected that the students know the basic structural features of the major biological macromolecules and understand the physical-chemical principles underlying the relationship between structure and function. The students should also understand the strategy, regulation, and bioenergetics of the central metabolic pathway. It is expected that the students apply the concepts developed in the lectures to the analysis of experimental results obtained in the laboratory classes and to problem solving.
Prerequisites
Strongly recommended a basic background of organic chemistry and general chemistry.
Subject matter
4. Summary of the lectures:
1- Introduction to Biochemistry (Biological structures, metabolic pathways, expression and transmission of genetic information).
2- The amino acids properties: peptide bonds - Classification and characteristics. Acid-base properties
3- Levels of protein structure organization: primary, secondary (alpha helices, beta sheets and other structural elements), tertiary and quaternary structures. Protein isolation, purification and sequencing.
4- Fibrous proteins (Keratin, silk, collagen) and globular protein (Myoglobin and hemoglobin)
5- Structure-function mechanisms in the hemoglobin.
6- Enzyme kinetics Inhibition and regulation.
7- Enzyme mechanisms: Lisozima, Ribonuclease, Carboxipeptidase, Quimotripsina.
8- Carbohydrates (Classification and function). Monosaccharides and structural and storage polysaccharides (cellulose, chitin, starch and glycogen). Glycoproteins (Proteoglycans and glycosylated proteins).
9- Biological Membranes: structure and dynamic. Biological transport: Passive-mediated transport, active-transport. Ion channels, Uniport, symport, and antiporte systems.
10- Nucleic acids (RNA and DNA): structure and function. Denaturation and Renaturation of nucleic acid. Restriction endonucleases: the chain- terminator method of DNA sequencing and Manual sequencing. Recombinant DNA technology. PCR and site-directed mutagenesis techniques.
11- Metabolism (Catabolism and anabolism) and Bioenergetics: Glycolysis, lactic and alcoholic fermentation, Citric acid Cycle, electron transport chain and oxidative phosphorylation.
12 – Photosynthesis.
Problem-solving sessions:
1 -Amino acid and Proteins – Acid-base properties
2 - Structure polypeptides and proteins.
3 - Enzyme kinetics
4 - Nucleic acids. Biological membranes.
5 – Metabolism
Laboratory sessions :
1 – Amino acid titrations
2 – Separation of biological compounds by chromatography
A – Gel filtration
B – Ionic Chromatography
3 – Enzymatic characterization of Fusarium graminearum beta-glucosidas
4 - Protein determination
Bibliography
Recommended textbooks
LEHNINGER PRINCIPLES OF BIOCHEMISTRY
Nelson, D.L., & Cox, M.M.
W.H. Freeman and Company, San Francisco, 5th ed. 2008/6th ed. 2013/7th ed. 2017
PRINCIPLES OF BIOCHEMISTRY
Voet, D., Voet, J.G. & Pratt, C.W.
John Wiley & Sons, Inc., New York, 4th ed. 2012
BIOCHEMISTRY
Lubert Stryer
W. H. Freeman and Company, San Francisco. 8th Ed. 2015
Teaching method
The course is organized in lectures, laboratory classes and workshops. The students must apply the concepts developed in the lectures to the analysis of the experimental results obtained in the laboratory and to problem solving in the workshops.
Evaluation method
Evaluation
The final evaluation is the result of a theoretical examination paper (80%) and a practical component (20%) that accounts for the laboratory work. The attendance to the laboratory classes is compulsory besides 70% of lectures (T and TP).